From:	onbehalfof+palaniswamy.jhsmp+gmail.com@manuscriptcentral.com on 
behalf of palaniswamy.jhsmp@gmail.com
Sent:	13 January 2015 07:49
To:	rankovic@kg.ac.rs
Subject:	Reminder: Invitation to review Manuscript WHSM-2014-0172 - Journal of 
Herbs, Spices & Medicinal Plants

13-Jan-2015

Dear Dr Branislv Rankovic:

Recently, I invited you to review the above manuscript, entitled 
"ANTILIPOXYGENASE, RADICAL SCAVENGING AND ANTIMICROBIAL ACTIVITIES OF LICHEN 
SPECIES OF GENUS HETERODERMIA (FAMILY PHYSCIACEAE)."  I have yet to hear from 
you about this.

This e-mail is simply a reminder to respond to the invitation to review.  I 
appreciate your help in accomplishing our goal of having an expedited 
reviewing process.  Please do not hesitate to contact me if I can be of any 
assistance.

To automatically register your response to the invitation to review, please 
click on the link below:

Agreed: 
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2

Declined: 
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Unavailable: 
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Once you have agreed to review the paper, you will be sent an email with a 
direct link to the scoresheet, which will be made available to you.

Sincerely,
Usha Palaniswamy
Journal of Herbs, Spices & Medicinal Plants Editorial Office 
palaniswamy.jhsmp@gmail.com


TITLE: ANTILIPOXYGENASE, RADICAL SCAVENGING AND ANTIMICROBIAL ACTIVITIES OF 
LICHEN SPECIES OF GENUS HETERODERMIA (FAMILY PHYSCIACEAE)

ABSTRACT:

The aim of the research is to explore antilipoxygenase, radical scavenging and 
antimicrobial activity of the ethyl acetate extract of the lichen species 
Heterodermia albicans, H.angustiloba, H.antillarum, H.diademata, H.flabellata, 
H.incana, H.isidiophora, H.pseudospeciosa of genus Heterodermia, family 
Physciaceae. All the species extract have shown concentration dependent 
lipoxygenase (LOX) inhibition. H.diademata and H.albicans inhibited 69.2% to 
86.64% lipoxygenase at a extract concentration between 120 to 200 µg/ml. 50% 
LOX inhibition was obtained by H.diademata, H.flabellata, H.antillarum and 
H.incana showed as IC50 value of 0.123, 0.153, 0.160, 0.150 mg/ml 
respectively. Which is smaller than the synthetic antioxidant BHA and BHT. 
Only the extract of lichen species H. diademata showed IC50 value 0.123 mg/ml 
is equal to the IC50 value of Indomethicin a standard LOX inhibitor. The LOX 
inhibition kinetics with respect to the lichen species extract resulted 
different mode of inhibition. H.albicans, H.antillarum showed competitive 
inhibition towards LOX, H.diademata showed uncompetitive inhibition. However, 
H.angustiloba, H.flabellata, H.incana, H.isidiophora and H.psedospeciosa 
showed non-competitive inhibition. All the species have shown ? 50% radical 
scavenging activity. The work also explored the antimicrobial activity of the 
extract of mentioned lichen species against microbes B.subtills, C.albicans, 
C.albidus, S.faecalis. The extract of the lichens H.diademata, H.angustiloba, 
H.albicans, H.isidiophora showed the strong antimicrobial activity against 
microorganisms at a concentration ranging from 0.232 mg/ml to 0.591 mg/ml. 
These results suggest that lichen species of the genus Heterodermia can be 
used as natural resource for lipoxygenase inhibitor with antimicrobial and 
radical scavenging properties.
