From:	JSCS : : Journal Manager [jscs@shd.org.rs]
Sent:	Wednesday, November 02, 2016 8:08 AM
To:	Branislav Rankovi?
Subject:	[JSCS 3940] Article Review Request


Dear Dr Rankovi?:

The Editorial Board of the Journal of the Serbian Chemical Society would be 
most grateful if you could referee the manuscript "Biological activities of 
Umbilicaria crustulosa (Ach.) Frey acetone extract,"which has been submitted 
for publication in the Journal. The submission's abstract is inserted below, 
and I hope that you will consider undertaking this important task for us.

Please log into the journal web site by 2016-11-09 to indicate whether you 
will undertake the review or not, as well as to access the submission and to 
record your review and recommendation.

The review itself is due 2016-11-30.

Submission URL:
http://www.shd-pub.org.rs/index.php/JSCS/reviewer/submission/1660?key=4cLH3b42

Thank you for considering this request.

Dr. Olgica Nedi?
INEP, Institute for the Application of Nuclear Energy, Zemun Belgrade, jscs-
bh@shd.org.rs



"Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract"

Abstract

This study reporting for the first time effect of U. crustulosa acetone 
extract on micronucleus distribution on human lymphocytes, effect on 
cholinesterase activity and antioxidant activity by the CUPRAC method.
Additionally, total phenolic compounds (TPC), antioxidant properties 
(estimated via DPPH, ABTS and TRP assays) as well as antibacterial activity 
against two Gram-positive and three Gram-negative bacteria were determined.
Acetone extract of U. crustulosa at concentration of 1 µg mL-1 and 2 µg
mL-1  decreased a frequency of micronuclei (MN) for 10.8 % and 16.8 %, 
respectively, acting higher or slightly less than a synthetic protector 
amifostine (AMF, WR-2721), (11.4 %, at concentration of 1 µg mL-1). Tested 
extract did not exhibit inhibition of cholinesterase activity as well as 
activity toward examined bacteria. The extract reduces the concentration of 
DPPH and ABTS radicals for 88.7 and 96.2 % (respectively). Values for total 
reducing power (TRP) and cupric reducing capacity (CUPRAC) were
0.6197±0.0166 µg ascorbic acid equivalents (AAE) per mg of dry extract, and 
19.7641±1.6546 µg Trolox equivalents (TE) per mg of dry extract, respectively. 
The total phenol content was 350.4188 ±14.587 µg galic acid equivalents (GAE) 
per mg of dry extract. The results of present study show that U. crustulosa 
acetone extract is a promising candidate for in vivo experiments considering 
antioxidant activity and protective effect on human lymphocytes. 

JSCS : : Biochemistry & Biotechnology Sub Editor 
____________________________________________________________
Journal of the Serbian Chemical Society
http://www.shd-pub.org.rs/index.php/JSCS
