From:	onbehalfof+mahady+uic.edu@manuscriptcentral.com on behalf of 
mahady@uic.edu
Sent:	03 March 2015 19:56
To:	rankovic@kg.ac.rs
Subject:	Pharmaceutical Biology - Invitation to Review Manuscript ID NPHB-2015-
0521

03-Mar-2015

Dear Professor Branislav Rankovic:

The above manuscript, entitled "Radical scavenging, prolyl endopeptidase 
inhibitory and antimicrobial potential of cultured Himalayan lichen Cetrelia 
olivetorum" with Dr Behera as contact author has been submitted to 
Pharmaceutical Biology.

I would be grateful if you would kindly agree to act as a reviewer for this 
paper.  The abstract appears at the end of this letter, along with the names 
of the authors.

Please let me know as soon as possible if you will be able to accept my 
invitation to review.  To do this please either click the appropriate link 
below to automatically register your reply with our online manuscript 
submission and review system, or e-mail me with your reply.

Agreed: 
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I realise that our expert reviewers greatly contribute to the high standards 
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Sincerely,

Gail B. Mahady, Ph.D.
Associate Professor
University of Illinois at Chicago


MANUSCRIPT DETAILS

TITLE: Radical scavenging, prolyl endopeptidase inhibitory and antimicrobial 
potential of cultured Himalayan lichen Cetrelia olivetorum

AUTHORS: Behera, Bhaskar; Savale, Swapnil; Pol, Chaitrali; Khare, Roshni; 
Verma, Neeraj; Gaikwad, Subhash; Mandal, Bapi

ABSTRACT: Context: Lichens are a great source of natural bioactive compounds 
which have traditionally been used to treat a variety of ailments.

Objective: To assess free radical scavenging, prolyl endopeptidase inhibitory 
and antimicrobial potential of a high altitude lichen species C. olivetorum.

Materials and Methods: Lichen C. olivetorum has been cultured in vitro and 
optimized culture conditions were implemented in bioreactor to obtain high 
quantity of biomass for the study of radical scavenging, PEP inhibitory and 
antimicrobial activities.

Results: MS medium supplemented with 3% glucose and 100ppb IBA supported 
biomass growth at flask level and yielded 5.095 g biomass. The methanol 
extract of C. olivetorum showed significant PEP inhibitory activity (17.33%) 
at 50 g/mL conc, in comparison to standard PEP inhibitor Z-Pro-prolinal 
(8.66%) at 20 g/mL conc. The lichen extract showed high antioxidant activities 
(> 89%) at 100 g/mL conc than the standard antioxidants. The MIC value of 
lichen extracts against tested microorganisms were obtained in the range of 50 
g to 104 g/mL and found to be more effective than erythromycin.

Discussion: MS medium containing IBA found to support maximum biomass 
production of C. olivetorum under bioreactor conditions. The cultured lichen 
biomass extract also showed PEP inhibitory, antioxidant and antimicrobial 
potential.

Conclusion: The present study indicates therapeutic potential of Himalayan 
lichen C. olivetorum against neurodegenerative diseases owing to its radical 
scavenging, PEP inhibitory and antimicrobial activities. Further it also 
encourages its commercial exploitation through mass culture and purification 
of its bioactive compounds may be used in pharmaceutical and neutraceutical 
supplements.



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